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STUDY OF THE THERMAL PROPERTIES AND MOLECULAR MASS OF POLYHYDROXYBUTYRATE PRODUCED BY RHODOCOCCUS EQUI

Pages: 9 – 15

Nadia Altaee, Ayad Fahdil, Kumar Sudesh and Emad Yousif

Abstract:

In the present study, the ability of Rhodococcus equi for polyhydroxyalkanoates (PHA) production was closely observed in the presence of crude palm kernel oil as a carbon source. Gas chromatography analysis proved that the biopolymer was a homopolymer that comprised of 38% polyhydroxybutyrate (PHB). The melting temperature (at 173°C) and glass transition temperature (at 2.79 °C) of PHA was determined using differential scanning calorimetry analysis, and the decomposition temperature (at 276°C) was determined by thermogravimetric analysis. The gel permeation chromatography revealed the average molecular weight ( ) to be at 6.42 KD, the number average weight ( ) at 373 KD, and the polydispersity ( / ) to be at 1.91. The present study demonstrated the use of an inexpensive substrate for PHB production by gram-positive bacteria that limited the study previously for the production of PHB polymer, which was analyzed by the polymer’s thermal properties and molecular mass.

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